Extraction and purification of C-phycocyanin from Spirulina platensis (CCC540)

被引：159

作者：

Kumar D. ^{[1
]}

Dhar D.W. ^{[1
]}

Pabbi S. ^{[1
]}

Kumar N. ^{[2
]}

Walia S. ^{[3
]}

机构：

[1] Centre for Conservation and Utilisation of Blue Green Algae, Indian Agricultural Research Institute, New Delhi

[2] Microbiology Department, Kurukshetra University, Kurukshetra

[3] Division of Agricultural Chemical, Indian Agricultural Research Institute, New Delhi

来源：

Indian Journal of Plant Physiology | 2014年 / 19卷 / 2期

关键词：

DEAE-Cellulose-11; Phycocyanin; Spirulina;

D O I：

10.1007/s40502-014-0094-7

中图分类号：

学科分类号：

摘要：

In this study a simple protocol was developed for purifying phycocyanin (PC) from Spirulina platensis (CCC540) by using ammonium sulphate precipitation, followed by a single step chromatography by using DEAE-Cellulose-11 and acetate buffer. Precipitation with 65 % ammonium sulphate resulted in 80 % recovery of phycocyanin with purity of 1.5 (A620/A280). Thro1ugh chromatography an 80 % recovery of phycocyanin with a purity of 4.5 (A620/A280) was achieved. In SDS_PAGE analysis, the purified PC showed the presence of two subunit α (16 kD) and β (17 kD). © 2014 The Author(s).

引用

页码：184 / 188

页数：4

共 33 条

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