CRISPR/Cas9 technology: applications in oocytes and early embryos

被引:0
|
作者
Yi-ran Zhang
Tai-lang Yin
Li-quan Zhou
机构
[1] Huazhong University of Science and Technology,Institute of Reproductive Health, Tongji Medical College
[2] Renmin Hospital of Wuhan University & Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development,Reproductive Medical Center
来源
Journal of Translational Medicine | / 21卷
关键词
CRISPR; Oocyte; Embryo; Development; Human germline genome editing;
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中图分类号
学科分类号
摘要
CRISPR/Cas9, a highly versatile genome-editing tool, has garnered significant attention in recent years. Despite the unique characteristics of oocytes and early embryos compared to other cell types, this technology has been increasing used in mammalian reproduction. In this comprehensive review, we elucidate the fundamental principles of CRISPR/Cas9-related methodologies and explore their wide-ranging applications in deciphering molecular intricacies during oocyte and early embryo development as well as in addressing associated diseases. However, it is imperative to acknowledge the limitations inherent to these technologies, including the potential for off-target effects, as well as the ethical concerns surrounding the manipulation of human embryos. Thus, a judicious and thoughtful approach is warranted. Regardless of these challenges, CRISPR/Cas9 technology undeniably represents a formidable tool for genome and epigenome manipulation within oocytes and early embryos. Continuous refinements in this field are poised to fortify its future prospects and applications.
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