Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

被引：0
|
作者：
Natalay Kouprina
Vladimir Larionov
机构：
[1] National Cancer Institute,Developmental Therapeutics Branch
来源：
Chromosoma | 2016年 / 125卷
关键词：
Transformation-associated recombination; TAR cloning; Human artificial chromosome; HAC; Synthetic biology;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Transformation-associated recombination (TAR) cloning represents a unique tool for isolation and manipulation of large DNA molecules. The technique exploits a high level of homologous recombination in the yeast Sacharomyces cerevisiae. So far, TAR cloning is the only method available to selectively recover chromosomal segments up to 300 kb in length from complex and simple genomes. In addition, TAR cloning allows the assembly and cloning of entire microbe genomes up to several Mb as well as engineering of large metabolic pathways. In this review, we summarize applications of TAR cloning for functional/structural genomics and synthetic biology.
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页码：621 / 632
页数：11
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