LncRNA LINC01197 inhibited the formation of calcium oxalate-induced kidney stones by regulating miR-516b-5p/SIRT3/FOXO1 signaling pathway

被引：0
作者：
Junhua Xi
Yang Chen
Junfeng Jing
Wei Qi
Yanbing Zhang
机构：
[1] The Second People’s Hospital of Hefei (Hefei Hospital Affiliated to Anhui Medical University),Department of Urology
[2] The Fifth School of Clinical Medical of Anhui Medical University,Institute of Urology
[3] Anhui Medical University,undefined
来源：
Cell and Tissue Research | 2023年 / 392卷
关键词：
LINC01197; Sirtuin 3; miR-516b-5p; FOXO1; Calcium oxalate; Kidney stone;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Long non-coding RNA (lncRNA) plays a key role in the regulation of calcium oxalate (CaOx) crystals-induced kidney stone formation and deposition. The purpose of this study is to study the effect of lncRNA LINC01197 on CaOx-induced kidney stone formation and the underlying mechanism. Crystal cell adhesion in HK-2 cells was evaluated by analyzing Ca2+ concentration. Apoptosis was detected by flow cytometry. The RT-qPCR and western blot were used to detect the mRNA and protein expression. Patients with kidneys stones showed down-regulated LINC01197 and SIRT3 expression, and up-regulated miR-516b-5p expression. LINC01197 knockdown promoted CaOx-induced cell adherence and cell apoptosis, increased Bax, decreased Bcl-2 expression. Luciferase reporter assay showed that SIRT3 expression was promoted by LINC01197 competing binds to miR-516b-5p. In addition, LINC01197 expression was promoted by SIRT3/FOXO1 overexpression, and could be reversed by FOXO1 knockdown. In conclusion, the present study revealed that lncRNA LINC01197 inhibited CaOx-induced kidney stones formation by regulating the miR-516b-5p/SIRT3/FOXO1 signaling pathway.
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页码：553 / 563
页数：10
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  • [1] Albert A(2017)Expression of heterologous oxalate decarboxylase in HEK293 cells confers protection against oxalate induced oxidative stress as a therapeutic approach for calcium oxalate stone disease J Enzyme Inhib Med Chem 32 426-433
  • [2] Tiwari V(2018)Kidney stone disease: an update on current concepts Adv Urol 2018 3068365-39
  • [3] Paul E(2014)FOXM1: a key oncofoetal transcription factor in health and disease Semin Cancer Biol 29 32-1705
  • [4] Ganesan D(2019)FOXO1 degradation via G9a-mediated methylation promotes cell proliferation in colon cancer Nucleic Acids Res 47 1692-148
  • [5] Ayyavu M(2018)FOXO1 inhibits tumor cell migration via regulating cell surface morphology in non-small cell lung cancer cells Cell Physiol Biochem 48 138-295
  • [6] Kujur R(2009)Foxo1 increases pro-inflammatory gene expression by inducing C/EBPbeta in TNF-alpha-treated adipocytes Biochem Biophys Res Commun 378 290-2367
  • [7] Ponnusamy S(2020)Clinical significance of CA 19.9 and LINC01197 in pancreatic cancer Eur Rev Med Pharmacol Sci 24 2358-86659
  • [8] Shanmugam K(2016)Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1 Oncotarget 7 86648-378
  • [9] Saso L(2019)LncRNA-miRNA-mRNA expression variation profile in the urine of calcium oxalate stone patients BMC Med Genomics 12 57-2271
  • [10] Govindan Sadasivam S(2019)FOXO1-regulated lncRNA LINC01197 inhibits pancreatic adenocarcinoma cell proliferation by restraining Wnt/β-catenin signaling J Exp Clin Cancer Res 38 179-19
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