α-Farnesene production from lipid by engineered Yarrowia lipolytica

被引:0
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作者
Yinghang Liu
Zhaoxuan Wang
Zhiyong Cui
Qingsheng Qi
Jin Hou
机构
[1] Shandong University,State Key Laboratory of Microbial Technology
[2] Chinese Academy of Sciences,CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology
来源
Bioresources and Bioprocessing | / 8卷
关键词
α-Farnesene; Erg12; Oleic acid; Waste cooking oils;
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中图分类号
学科分类号
摘要
Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well as improve the economic value of wasted oils and fats. Yarrowia lipolytica is a non-conventional oleaginous yeast and can grow on a variety of hydrophobic substrates. In this study, we explored its ability to synthesize α-farnesene, an important sesquiterpene, using lipid feedstock. Based on the α-farnesene production strain, we constructed previously, we identified that Erg12 was the key limiting factor to further increase the α-farnesene production. The α-farnesene production was improved by 35.8% through increasing the copy number of ERG12 and FSERG20 on oleic acid substrate. Expression of heterologous VHb further improved α-farnesene production by 12.7%. Combining metabolic engineering with the optimization of fermentation conditions, the α-farnesene titer and yield reached 10.2 g/L and 0.1 g/g oleic acid, respectively, in fed-batch cultivation. The α-farnesene synthesis ability on waste cooking oil and other edible oils were also explored. Compared with using glucose as carbon source, using lipid substrates obtained higher α-farnesene yield and titer, but lower by-products accumulation, demonstrating the advantage of Y. lipolytica to synthesize high value-added products using lipid feedstock.
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