Distribution of hepatitis B virus subgenotype F2a in São Paulo, Brazil

被引：3
作者：
Alvarado-Mora M.V. [1 ]
Botelho-Lima L.S. [1 ]
Santana R.A. [2 ]
Sitnik R. [2 ]
Ferreira P.A. [3 ]
Do Amaral Mello F. [4 ]
Mangueira C.P. [2 ]
Carrilho F.J. [1 ]
R Rebello Pinho J. [1 ,2 ]
机构：
[1] Department of Gastroenterology, School of Medicine, University of São Paulo, São Paulo, SP
[2] Albert Einstein Diagnostic Medicine, São Paulo, SP
[3] Federal University of São Paulo - UNIFESP, São Paulo, SP
[4] Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro
来源：
BMC Research Notes | / 6卷 / 1期
基金：
巴西圣保罗研究基金会;
关键词：
Bayesian analysis; Brazil; Hepatitis B; Subgenotype F2a;
D O I：
10.1186/1756-0500-6-423
中图分类号：
学科分类号：
摘要：
Background: HBV genotype F is primarily found in indigenous populations from South America and is classified in four subgenotypes (F1 to F4). Subgenotype F2a is the most common in Brazil among genotype F cases. The aim of this study was to characterize HBV genotype F2a circulating in 16 patients from São Paulo, Brazil. Samples were collected between 2006 and 2012 and sent to Hospital Israelita Albert Einstein. A fragment of 1306 bp partially comprising HBsAg and DNA polymerase coding regions was amplified and sequenced. Viral sequences were genotyped by phylogenetic analysis using reference sequences from GenBank (n=198), including 80 classified as subgenotype F2a. Bayesian Markov chain Monte Carlo simulation implemented in BEAST v.1.5.4 was applied to obtain the best possible estimates using the model of nucleotide substitutions GTR+G+I. Findings. It were identified three groups of sequences of subgenotype F2a: 1) 10 sequences from São Paulo state; 2) 3 sequences from Rio de Janeiro and one from São Paulo states; 3) 8 sequences from the West Amazon Basin. Conclusions: These results showing for the first time the distribution of F2a subgenotype in Brazil. The spreading and the dynamic of subgenotype F2a in Brazil requires the study of a higher number of samples from different regions as it is unfold in almost all Brazilian populations studied so far. We cannot infer with certainty the origin of these different groups due to the lack of available sequences. Nevertheless, our data suggest that the common origin of these groups probably occurred a long time ago. © 2013 Alvarado-Mora et al.; licensee BioMed Central Ltd.
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