Sequence specific 1H, 13C, and 15N resonance assignments of Hahellin from Hahella chejuensis, a putative member of the βγ-crystallin superfamily

被引:0
作者
Atul K. Srivastava
Yogendra Sharma
K. V. R. Chary
机构
[1] Tata Institute of Fundamental Research,Department of Chemical Sciences
[2] Center for Cellular and Molecular Biology (CCMB),undefined
来源
Biomolecular NMR Assignments | 2008年 / 2卷
关键词
βγ-Crystallins; Hahellin; Sequence specific resonance assignment;
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摘要
The sequence specific 1H, 13C, and 15N resonance assignments of Hahellin, a putative member of βγ-crystallin family, from Hahella Chejuensis, have been accomplished by NMR spectroscopy. The resonance assignments reveal that the protein adopts predominantly a β-sheet conformation as in the case of βγ-crystallin folds.
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页码:151 / 153
页数:2
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共 47 条
  • [1] Atreya HS(2000)A tracked approach for automated NMR assignments in proteins (TATAPRO) J Biomol NMR 17 125-136
  • [2] Sahu SC(1993)Methodological advances in protein NMR Acc Chem Res 22 131-138
  • [3] Chary KV(2004)Transparency and non-refractive functions of crystallins—a proposal Exp Eye Res 79 809-816
  • [4] Govil G(2004)Ageing and vision: structure, stability and function of lens crystallins Prog Biophys Mol Biol 86 407-485
  • [5] Bax A(2001)Lens crystallins and their microbial homologs: structure, stability, and function Crit Rev Biochem Mol Biol 36 435-499
  • [6] Grzesiek S(2005)Genomic blueprint of Nucleic Acids Res 33 7066-7073
  • [7] Bhat SP(2007), a marine microbe producing an algicidal agent Biochemistry 46 12298-12307
  • [8] Bloemendal H(1992)Caulollins from J Am Chem Soc 113 5490-5492
  • [9] de Jong W(1997), a pair of partially unstructured proteins of βγ-crystallin superfamily, gain structure upon binding calcium Proc Natl Acad Sci USA 94 3229-3234
  • [10] Jaenicke R(1997)An efficient triple resonance experiment using carbon-13 isotopic mixing for determining sequence-specific resonance assignment of isotopically-enriched proteins J Mol Biol 271 645-655