Genistein-induced proteome changes in the human endometrial carcinoma cell line, Ishikawa

被引：0
作者：
Konstantakopoulos N. [1 ,2 ,8 ]
Larsen M.R. [3 ]
Campbell I.G. [4 ]
Quinn M.A. [1 ,2 ]
Baker M.S. [5 ]
Georgiou H.M. [2 ,6 ]
Rice G.E. [1 ,7 ]
机构：
[1] Gynaecological Cancer Research Centre, Royal Women's Hospital, Carlton, VIC
[2] Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, VIC
[3] Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M
[4] VBCRC Cancer Genetics Laboratory, Centre for Cancer Genomics and Predictive Medicine, Peter MacCallum Cancer Centre, East Melbourne, VIC, St. Andrews Place
[5] Australian Proteome Analysis Facility, Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW
[6] Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, VIC
[7] Translational Proteomics, Baker Medcal Research Institute, Melbourne, VIC
[8] Gynaecological Cancer Research Centre, Royal Women's Hospital, Carlton
来源：
Clinical Proteomics | 2006年 / 2卷 / 3-4期
关键词：
Endometrial cancer; Genistein; Phytoestrogen; Proteomics; Tumor suppression;
D O I：
10.1007/BF02752498
中图分类号：
学科分类号：
摘要：
Epidemiological studies have shown that Asian populations display a lower incidence of hormone-dependant cancers, cardiovascular disease, osteoporosis, and menopausal ailments compared to Western societies. Available data support the proposal that lower incidence is associated with the high dietary consumption of isoflavones, such as genistein. This study used two-dimensional electrophoresis to characterize the effect of genistein on the proteome of an endometrial tumor cell model, namely the Ishikawa cell line. Proteome maps displaying approx 1800 proteins were obtained from cells treated with vehicle or genistein at physiologically attainable concentrations of 0.5, 5, or 50 μM or supra-physiological concentration, 500 μM. The effects of genistein on protein expression were characterized using image analysis software. A total 65 protein spots displayed a significant decrease in expression and 32 proteins displayed a significant increase in expression. Of these protein spots, 29 were randomly selected for characterization by matrix assisted laser desorption/ionization tandem mass spectrometry, yielding 18 different proteins. This type of analysis enabled the characterization of a wide range of cellular proteins and allowed for the identification of functional and biochemical pathways that may be regulated or affected by genistein, including cellular transcription, cell proliferation, stress response, or modulation of oncogenic pathways. © 2006 Humana Press Inc.
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页码：153 / 167
页数：14
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