Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization

被引：1
|
作者：
Sophie Trouvelot
Diederik van Tuinen
Mohamed Hijri
V. Gianinazzi-Pearson
机构：
[1] Laboratoire de Phytoparasitologie INRA/CNRS,
[2] Centre de Microbiologie du Sol et de l'Environnement,undefined
[3] INRA,undefined
[4] BV 1540,undefined
[5] F-21034 Dijon Cedex,undefined
[6] France e-mail : gianina@epoisses.inra.fr,undefined
[7] Fax: +333-80-63-32-63,undefined
来源：
Mycorrhiza | 1999年 / 8卷
关键词：
Key words Arbuscular mycorrhiza; Spore nuclei; Fluorescence in situ hybridization; Ribosomal DNA loci;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
 Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.
引用
收藏
页码：203 / 206
页数：3
相关论文
共 50 条
  • [1] Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization
    Trouvelot, S
    van Tuinen, D
    Hijri, M
    Gianinazzi-Pearson, V
    MYCORRHIZA, 1999, 8 (04) : 203 - 206
  • [2] Rapid fluorescence in situ hybridization on interphasic nuclei to discriminate between homozygous and heterozygous transgenic mice
    Paris, D
    Toyama, K
    Megarbane, A
    Casanova, M
    Sinet, PM
    London, J
    TRANSGENIC RESEARCH, 1996, 5 (06) : 397 - 403
  • [3] VISUALIZATION OF MITOTIC CHROMOSOMES IN FILAMENTOUS FUNGI BY FLUORESCENCE STAINING AND FLUORESCENCE IN-SITU HYBRIDIZATION
    TAGA, M
    MURATA, M
    CHROMOSOMA, 1994, 103 (06) : 408 - 413
  • [4] Comparison of ribosomal DNA sites in Lolium species by fluorescence in situ hybridization
    Thomas, HM
    Harper, JA
    Meredith, MR
    Morgan, WG
    Thomas, ID
    Timms, E
    King, IP
    CHROMOSOME RESEARCH, 1996, 4 (07) : 486 - 490
  • [5] Fluorescence in situ hybridization of freshwater fungi
    Baschien, C
    Manz, W
    Neu, TR
    Szewzyk, U
    INTERNATIONAL REVIEW OF HYDROBIOLOGY, 2001, 86 (4-5) : 371 - 381
  • [6] Detection of a variable number of ribosomal DNA loci by fluorescent in situ hybridization in Populus species
    Prado, EA
    FaivreRampant, P
    Schneider, C
    Darmency, MA
    GENOME, 1996, 39 (05) : 1020 - 1026
  • [7] High resolution mapping of ribosomal DNA in early mouse embryos by fluorescence in situ hybridization
    Romanova, L
    Korobova, F
    Noniashvilli, E
    Dyban, A
    Zatsepina, O
    BIOLOGY OF REPRODUCTION, 2006, 74 (05) : 807 - 815
  • [8] In situ hybridization of ribosomal DNA to rose chromosomes
    Ma, Y
    IslamFaridi, MN
    Crane, CF
    Ji, Y
    Stelly, DM
    Price, HJ
    Byrne, DH
    JOURNAL OF HEREDITY, 1997, 88 (02) : 158 - 161
  • [9] Fluorescence microscopy and fluorescence in situ hybridization of Entamoeba histolytica nuclei to analyse mitosis and the localization of repetitive DNA
    Willhoeft, U
    Tannich, E
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 105 (02) : 291 - 296
  • [10] Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei for DNA fluorescence in situ hybridization
    Xiaozhu Wang
    Shin-ichiro Takebayashi
    Evans Bernardin
    David M. Gilbert
    Ravindran Chella
    Jingjiao Guan
    Biomedical Microdevices, 2012, 14 : 443 - 451
12345