Filter paper saturated by urine sample in metabolic disorders detection by proton magnetic resonance spectroscopy

被引:0
作者
Hélène Blasco
Marie-Ange Garrigue
Aymeric De Vos
Catherine Antar
François Labarthe
François Maillot
Christian R Andres
Lydie Nadal-Desbarats
机构
[1] Inserm U930,Laboratoire de Biochimie et Biologie Moléculaire
[2] CNRS 2448,Université François
[3] Université François-Rabelais,Rabelais
[4] CHRU de Tours,Service de Pédiatrie R
[5] Hôpital Clocheville,Service de médecine interne
[6] CHRU de Tours,undefined
[7] Inserm U921,undefined
[8] CHRU de Tours,undefined
[9] Inserm U921,undefined
来源
Analytical and Bioanalytical Chemistry | 2010年 / 396卷
关键词
Proton magnetic resonance spectroscopy; Inborn errors of metabolism; Urinary metabolite; Filter paper;
D O I
暂无
中图分类号
学科分类号
摘要
NMR spectroscopy of urine samples is able to diagnose many inborn errors of metabolism (IEM). However, urinary metabolites have a poor stability, requiring special care for routine analysis (storage of urine at −20 or −80 °C, fast transport). The aim of our study was to investigate the reliability of dried urine filter paper for urine storage and transport and to evaluate the ability of NMR to detect several IEM using this method. Urine samples from five healthy subjects were analyzed by 1H NMR following different storage conditions (−20 vs 4 °C vs dried on filter paper) and at different time points (24 h, 48 h, 96 h, and 7 days). Urine pattern of fresh urine was considered as a reference. We analyzed the conservation of some amino acids and organic acids using Bland and Altman plot with intraclass correlation coefficient determination. Then, we evaluated the use of filter paper to detect four different IEM (methylmalonic and isovaleric acidurias, ornithine transcarbamylase deficiency, and cystinuria). Analysis of urine samples from healthy subjects revealed a high stability of studied molecules (ICC > 0.8) even after 7 days of storage on filter paper. Moreover, an excellent preservation of metabolites specifically accumulated in IEM was observed when analysis of dried urine filter paper was compared to fresh urine (coefficient of variation < 15%). This preliminary study demonstrates that storage of dried urine on filter paper is reliable for 1H NMR spectroscopy analysis. Preservation of urine molecules over time using that method is convenient for routine clinical practice.
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页码:1205 / 1211
页数:6
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