Determination of Phytate in Urine by High-Performance Liquid Chromatography–Mass Spectrometry

被引：0

作者：

J. Perelló

B. Isern

J.A. Muñoz

M. Valiente

F. Grases

机构：

[1] Institute of Health Sciences Research (IUNICS),Laboratory of Renal Lithiasis Research

[2] University of the Balearic Islands,Grup de Tècniques de Separació en Química (GTS), Química Analítica

[3] Departament de Química,undefined

来源：

Chromatographia | 2004年 / 60卷

关键词：

Column liquid chromatography; Mass spectrometric detection; Phytate; Human urine samples;

D O I：

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学科分类号：

摘要：

An indirect method is described for determination of phytate in human urine. The method is based on hydrolysis of the phytate and determination of myo-inositol, one of the hydrolysis products. Chromatographic separations were performed on an Aminex HPX-87C column with Milli-Q water as mobile phase; 5 mM ammonium acetate was added post-column. The detector counted positive ions by monitoring m/z=198, which corresponds to the adduct of myo-inositol with the ammonium cation. The relative standard deviations obtained for standards containing 0.5, 1, and 1.5 mg L−1 phytate were 4.1, 3.0, and 2.7% respectively (n=5). The limit of detection was 60 μg L−1. Different urine samples were analyzed both by this method and by an alternative analytical method based on GC–MS. The results from both methods were comparable.

引用

页码：265 / 268

页数：3