Studying the Genetic Diversity of the Cause of Rice Blast Pyricularia oryzae Cav. on the Set of Molecular and Morphological Features

被引:0
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作者
D. V. Nartymov
E. V. Dubina
M. G. Ruban
Yu. V. Aniskina
S. V. Garkusha
I. A. Shilov
N. S. Velishaeva
O. S. Kolobova
N. K. Istomin
机构
[1] Federal Research Center for Rice,
[2] Trubilin Kuban State Agrarian University,undefined
[3] All-Russia Research Institute of Agricultural Biotechnology,undefined
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关键词
genetic biodiversity; phytopathogenic fungi; Cav. (blast); pathotype; strains; SSR loci; PCR; DNA passports;
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摘要
On the basis of molecular-genetic approaches, the study of the biodiversity of the phytopathogenic fungus Pyricularia oryzae Cav. in the south of Russia and the classification of the morphological and culture characteristics of the pathogen is carried out. Between 2020 and 2022 monitoring was carried out and 24 strains of the pathogen were isolated from the affected herbarium material collected from the fields of five ecological rice growing zones of Krasnodar krai (Krasnoarmeisky, Kalininsky, Abinsky, Slavyansky districts, Krasnodar). Using the multiplex PCR system developed by us based on fragment analysis, five genotypes are identified among the studied Pyricularia oryzae Cav. strains over the entire period of the project, each of which is characterized by a unique genetic profile. Their DNA passports are compiled. The studied isolates of the causative agent of blast are characterized by morphological and culture characteristics. Six morphotypes of the pathogen are identified.
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页码:551 / 557
页数:6
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