Osteoinductive Properties of Secretome of Human Mesenchymal Stem Cells, Obtained with Automatic Cell Culture System

被引：2
作者：
Aleksandrova S.A. [1 ]
Nashchekina Y.A. [1 ]
Nadezhdin S.V. [2 ]
Vasiliev S.A. [3 ]
Savchenko R.R. [3 ]
Pokrovskaya L.A. [4 ]
Blinova M.I. [1 ]
Mikhailova N.A. [1 ]
Khotin M.G. [1 ]
机构：
[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg
[2] Belgorod National Research University, Belgorod
[3] Scientific Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk
[4] National Research Tomsk State University, Tomsk
来源：
Cell and Tissue Biology | 2020年 / 14卷 / 6期
基金：
俄罗斯基础研究基金会;
关键词：
automatic cell culture system; cell line FetMSC; mesenchymal stem cells of human bone marrow; osteogenic differentiation; Runx2; secretome; YAP1;
D O I：
10.1134/S1990519X20060024
中图分类号：
学科分类号：
摘要：
Abstract: The secretome of human mesenchymal stem cells (MSCs) after osteogenic differentiation of FetMSC cells in vitro was obtained to develop tissue engineering technologies for bone tissue. For standard secretome samples a large number of FetMSC cells (7 × 108 cells) were cultured in serum-free conditioned medium (SFCM). All steps of cell processing were performed in the CompacT SelecT automatic system (Sartorius, United Kingdom). The SFCM was concentrated by ultrafiltration, then subjected to dialysis and dried in a vacuum rotary evaporator. The osteoinductive properties of SFCM concentrate (SFCMC) were tested on FetMSC cells by means of adding it to the growth medium at different concentrations. There were no changes in cell morphology during cultivation in the presence of SFCMC. Analysis of the transcription factors Runx2 and YAP1 (markers of osteogenic differentiation) expression using immunofluorescence staining and real-time polymerase chain reaction (RT-PCR) showed an increase in their level of expression in the presence of SFCMC. These findings demonstrate that it is possible to use SFCMC obtained from osteogenic MSCs to induce differentiation of other (undifferentiated) MSCs. The results are useful for the development of cell therapy products for bone restoration based on MSCs secretome. © 2020, Pleiades Publishing, Ltd.
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页码：492 / 500
页数：8
相关论文
共 19 条
  • [1] Assoni A., Coatti G., Valadares M.C., Beccari M., Gomes J., Pelatti M., and Zatz, M., Different donors mesenchymal stromal cells secretomes reveal heterogeneous profile of relevance for therapeutic use, Stem Cells Dev., 26, (2017)
  • [2] Baglio S.R., Pegtel D.M., Baldini N., Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy, Front. Phys, 3, (2012)
  • [3] Caplan A.I., Principles of Regenerative Medicine, (2019)
  • [4] Da Silva Meirelles L., Fontes A.M., Covas D.T., Caplan A.I., Mechanisms involved in the therapeutic properties of mesenchymal stem cells, Cyt. Growth Factor Rev, 20, (2009)
  • [5] Deev R.V., Drobyshev A.Y., Bozo I.Y., Isaev A.A., Ordinary and activated bone grafts: Applied classification and the main features, Biomed. Res. Int., (2015)
  • [6] Ivanova G., Pereira T., Caseiro A.R., Georgieva P., Mauricio A.C., Metabolomic and proteomic analysis of the mesenchymal stem cells’ secretome, in Metabolomics—Fundamentals and Applications, InTechIntechOpen, Ebook.
  • [7] Kim J.M., Kim J., Kim Y.H., Kim K.T., Ryu S.H., Lee T.G., Suh P.G., Comparative secretome analysis of human bone marrow-derived mesenchymal stem cells during osteogenesis, J. Cell. Phys, 228, (2013)
  • [8] Komori T., Signaling networks in RUNX2-dependent bone development, J. Cell Biochem, 112, (2011)
  • [9] Krylova T.A., Koltsova A.M., Zenin V.V., Musorina A.S., Yakovleva T.K., Poljanskaya G.G., Comparative characteristics of new lines of mesenchymal stem cells derived from human embryonic stem cells, bone marrow, and foreskin, Cell Tissue Biol, 6, (2012)
  • [10] Kulterer B., Friedl G., Jandrositz A., Sanchez-Cabo F., Prokesch A., Paar C., Scheideler M., Windhager R., Preisegger K.H., Trajanoski Z., Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation, BMC Genomics, 8, (2007)
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