Cloning and expression of Tenebrio molitor antifreeze protein in Escherichia coli

被引：0
作者：
Chang-Wu Yue
Yi-Zheng Zhang
机构：
[1] Sichuan University,College of Life Science, Sichuan Key Laboratory of Molecular Biology & Biotechnology
[2] Central Laboratory of Zunyi Medical College,undefined
来源：
Molecular Biology Reports | 2009年 / 36卷
关键词：
Antifreeze protein; Gene cloning; Gene expression; Inclusion body;
D O I：
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中图分类号：
学科分类号：
摘要：
A novel antifreeze protein cDNA was cloned by RT-PCR from the larva of the yellow mealworm Tenebrio molitor. The coding fragment of 339 bp encodes a protein of 112 amino acid residues and was fused to the expression vectors pET32a and pTWIN1. The resulted expression plasmids were transformed into Escherischia coli strains BL21 (DE3), ER2566, and Origami B (DE3), respectively. Several strategies were used for expression of the highly disulfide-bonded β-helix-contained protein with the activity of antifreeze in different expression systems. A protocol for production of refolded and active T. molitor antifreeze protein in bacteria was obtained.
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页码：529 / 536
页数：7
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