A simple method for identification of S phase nuclei in Vicia faba root meristems using BrdUrd labeling and indirect immunofluorescence (comparison with 3H-thymidine incorporation)

被引：0
作者：
Justyna Polit
Janusz Maszewski
机构：
[1] University of Łódź,Department of Cytophysiology
来源：
Acta Physiologiae Plantarum | 2001年 / 23卷
关键词：
autoradiography; BrdUrd; H-thymidine; immunofluorescence; labeling index; root meristems; S-phase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A modified immunocytochemical method for identification of S-phase cells in root meristems is desribed. The technique combines incorporation of BrdUrd, rapid isolation of cell nuclei and indirect immunocytochemical detection of BrdUrd-labeled areas of chromatin using monoclonal anti-BrdUrd (I) and FITC-conjugated (II) antibodies. The labeling indexes calculated using 3H-thymidine autoradiography and BrdUrd-immunofluorescence in root meristems of Vicia faba subsp. minor were found comparable. However, the increased sensitivity of fluorescent staining reveals an enhanced complexity of visible structure emerging during successive periods of S-phase, allowing for discriminating not only quantitative but also qualitative aspects of nuclear labeling patterns characteristic for specific periods of DNA replication.
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页码：95 / 101
页数：6
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