Parameter estimation and dynamic control analysis of central carbon metabolism in Escherichia coli

被引：0
作者：
Wangyun Won
Changhun Park
Changhun Park
Sang Yup Lee
Kwang Soon Lee
Jinwon Lee
机构：
[1] Sogang University,Department of Chemical and Biomolecular Engineering
[2] Yonsei University Health System,Clinical Trials Center
[3] Korea Advanced Institute of Science and Technology,Department of Chemical and Biomolecular Engineering
来源：
Biotechnology and Bioprocess Engineering | 2011年 / 16卷
关键词：
metabolic control analysis; metabolic flux analysis; parameter estimation; central metabolism;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The central carbon metabolic pathway is the most important among metabolic pathways in all microorganisms since it produces energy and precursors for biosynthesis. In this study, a dynamic model for central carbon metabolism in Escherichia coli (E. coli) consisting of the phosphotransferase (PTS) system, glycolysis, pentose-phosphate pathway (PPP), and storage materials was obtained by ameliorating the model proposed by Chassagnole et al. (2002). In order to improve the performance of the model, principal parameters were estimated through the experimental measurements of intracellular concentrations of metabolites under transient conditions. Through dynamic metabolic control analysis (MCA), the tendencies of the metabolic fluxes at branch points were investigated, and the key parameters and enzyme kinetics that most dominantly affected the productivity of the desired metabolites were determined.
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页码：216 / 228
页数：12
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