Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells sing Comet–FISH

被引：3
作者：
Tirukalikundram S Kumaravel
Robert G Bristow
机构：
[1] University Health Network,Ontario Cancer Institute/Princess Margaret Hospital
[2] Covance Laboratories Ltd,Genetic and Molecular Toxicology
[3] University of Toronto,Departments of Radiation Oncology and Medical Biophysics
[4] Princess Margaret Hospital (University Health Network),Department of Radiation Oncology
来源：
Breast Cancer Research and Treatment | 2005年 / 91卷
关键词：
breast cancer; breast cancer cell lines; Comet assay; DNA breaks; fluorescent ; hybridization (FISH); genetic instability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A proportion of breast cancers acquire genetic alterations at 17q11.2–q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We describe a unique technique (Comet–FISH) in which we documented relative genetic instability at p53 and HER-2/neu gene loci within a panel of malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336). Furthermore, Comet–FISH data were consistent with preferential repair of the p53 locus following gentoxic insult and suggest that this assay may be quite useful for the study of genetic instability.
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页码：89 / 94
页数：5
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[1]  
Keen JC(2003)The biology of breast carcinoma Cancer 97 825-833
[2]  
Davidson NE(2000)Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review J Natl Cancer Inst 92 874-897
[3]  
Berwick M(1994)Mutagen sensitivity as a marker of cancer risk Cancer Detect Prev 18 299-303
[4]  
Vineis P(1999)Genetic instability and darwinian selection in tumours Trends Cell Biol 9 M57-M60
[5]  
Spitz MR(2003)Roles of BRCA1 and BRCA2 in homologous recombination, DNA replication fidelity and the cellular response to ionizing radiation Oncogene 22 5784-5791
[6]  
Hsu TC(2002)Cancer susceptibility and the functions of BRCA1 and BRCA2 Cell 108 171-182
[7]  
Cahill DP(2003)The Oncologist 8 307-325
[8]  
Kinzler KW(1995) gene and protein in breast cancer 2003: biomarker and target of therapy Cancer Res 55 646-651
[9]  
Vogelstein B(2003)p53 abnormalities and genomic instability in primary human breast carcinomas Hum Mutat 21 301-306
[10]  
Lengauer C(2002)TP53 mutations in familial breast cancer: functional aspects Int J Biochem Cell Biol 34 1382-1394
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