Biofilm and fluoroquinolone resistance of canine Escherichia coli uropathogenic isolates

被引：25
作者：
Oliveira M. [1 ]
Dias F.R. [1 ]
Pomba C. [1 ]
机构：
[1] CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisbon
来源：
BMC Research Notes | / 7卷 / 1期
关键词：
Biofilm; Dogs; Escherichia coli; Fluoroquinolone resistance; Urinary tract infection;
D O I：
10.1186/1756-0500-7-499
中图分类号：
学科分类号：
摘要：
Escherichia coli is the most common uropathogen involved in urinary tract infection (UTI). Virulence of strains may differ, and may be enhanced by antimicrobial resistance and biofilm formation, resulting in increased morbidity and recurrent infections. The aim of this study was to evaluate the in vitro biofilm forming capacity of E. coli isolates from dogs with UTI, by using fluorescent in situ hybridization, and its association with virulence genes and antimicrobial resistance. Findings. The proportion of biofilm-producing isolates significantly increased with the length of incubation time (P < 0.05). Biofilm production was significantly associated with fluoroquinolone resistance at all incubation time points and was independent of the media used (P < 0.05). Biofilm production was not associated with cnf1, hly, pap and sfa genes (P > 0.05), but was significantly associated with afa, aer and the β-lactamase genes (P < 0.05). Conclusions: To the best of our knowledge, this is the first report showing significant association between biofilm production and fluoroquinolone resistance in E. coli isolates from dogs with UTI. Biofilm formation may contribute to UTI treatment failure in dogs, through the development of bacterial reservoirs inside bladder cells, allowing them to overcome host immune defenses and to establish recurrent infections. © 2014 Oliveira et al.; licensee BioMed Central Ltd.
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