Isolation and characterization of microsatellite loci in Sorbus aria (Rosaceae)

被引：0

作者：

E. A. González-González

M. A. González-Pérez

E. Rivero

P. A. Sosa

机构：

[1] Universidad de Las Palmas de Gran Canaria,Departamento de Biología

来源：

Conservation Genetics Resources | 2010年 / 2卷

关键词：

Microsatellite; Canary Islands; Genetic diversity; Triploid;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Sorbus aria (L) Crantz (Common Whitebeam) is native to Europe, east of the Balkans and in North Africa; it is also present in the Canary Islands. To evaluate the genetic diversity in natural populations of this vulnerable species, nine novel polymorphic microsatellite markers were isolated from enriched libraries. Microsatellite loci were screened in 97 individuals from La Palma (Canary Islands) and Sierra Nevada (Granada, Spain). Examination of the microsatellite profiles shows that S. aria individuals have up to three alleles per locus. The cloned sequences in microsatellite loci confirmed the polyploidy status of the plants. The number of alleles ranged from 5 to 14 per locus. The phenotype diversities across loci (H′T) ranging from 0.653 to 0.847.

引用

页码：341 / 343

页数：2

共 25 条

[1]

Campbell CS(1995)Phylogenetic relationship in Am J Bot 82 903-918

[2]

Donoughue MJ(2007) (Rosaceae): evidence from sequences of internal transcribed spacers of nuclear ribosomal DNA and its congruence with morphology Bot J Linn Soc 154 291-304

[3]

Baldwin BG(1987)Parentage of endemic Phytochem Bull Bot Soc Amer 19 11-15

[4]

Wojciechowski MF(2000) L (Rosaceae) species in the British Isles: evidence from plasmid DNA Genome 43 439-448

[5]

Chester M(2002)A rapid DNA isolation procedure for small quantities of fresh leaf tissue Theor Appl Genet 105 953-963

[6]

Cowan RS(2006)Isolation, characterization and inheritance of microsatellite loci in alpine and western larch Heredity 97 296-303

[7]

Fay MF(1991)The origin of intermediate species of the genus Syst Bot 16 376-394

[8]

Rich TCG(undefined)Simple allelic-phenotype diversity and differentiation statistics for allopolyploids undefined undefined undefined-undefined

[9]

Doyle JJ(undefined)A synopsis of genera in undefined undefined undefined-undefined

[10]

Doyle JL(undefined) (Rosaceae) undefined undefined undefined-undefined

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