Incomplete paternal inheritance of chloroplast DNA recognized in Chamaecyparis obtusa using an intraspecific polymorphism of the trnD-trnY intergenic spacer region

被引：0

作者：

S. Shiraishi

H. Maeda

T. Toda

K. Seido

Y. Sasaki

机构：

[1] Laboratory of Silviculture,

[2] Faculty of Agriculture,undefined

[3] Kyushu University,undefined

[4] Hakozaki,undefined

[5] Higashi-ku,undefined

[6] Fukuoka 812-8581,undefined

[7] Japan e-mail: sushi@agr.kyushu-u.ac.jp Fax: +81926422872 Present address: H. Maeda,undefined

[8] Nagasaki Prefectural Office,undefined

[9] Edo,undefined

[10] Nagasaki 850-0861,undefined

[11] Japan,undefined

[12] Kyushu Regional Breeding Office,undefined

[13] Forest Tree Breeding Center,undefined

[14] Nishigooshi,undefined

[15] Kumamoto 861-1102,undefined

[16] Japan,undefined

[17] Yamanashi Forestry and Forest Products Research Institute,undefined

[18] Masuho,undefined

[19] Yamanashi 400-0502,undefined

[20] Japan,undefined

[21] Oita Prefectural Forest Experiment Station,undefined

[22] Hita,undefined

[23] Oita 877-1363,undefined

[24] Japan,undefined

来源：

Theoretical and Applied Genetics | 2001年 / 102卷

关键词：

Keywords Biparental inheritance; Paternal inheritance; Heteroplasmy; Chloroplast DNA; Chamaecyparis; PCR-SSCP;

D O I：

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学科分类号：

摘要：

Using a fluorescence-based PCR-SSCP (single-strand conformation polymorphism), we verified imperfectibility in the paternal inheritance of chloroplast DNA (cpDNA) in Chamaecyparisobtusa (Cupressaceae) controlled crosses. An intraspecific sequence polymorphism of the intergenic spacer region between the trnD and trnY genes was utilized as a molecular marker. Of 361 progenies, in which the cpDNA haplotypes of their female and male parents were different, 352 (97.5%) possessed the same haplotypes as their male parents, and nine (2.5%) the same haplotypes as their female parents. The parentage of the nine progenies with female parental types was diagnosed using DNA fingerprinting based on fluorescence-based RAPD profiles. Their parentage showed convincing evidence of the low frequency of maternal inheritance. Moreover, heteroplasmy was observed in the open-pollinated seeds collected in a seed orchard. The confirmation of maternal plastid transmission in the full-sib families and the observation of heteroplasmy in seeds reveal that the paternal inheritance of cpDNA is not an exclusive phenomenon and that the mode of its inheritance is biparental in C. obtusa.

引用

页码：935 / 941

页数：6