Exploring the genetic diversity and recombination analysis of Citrus tristeza virus isolates prevalent in Northeast India

被引：3
作者：
Gautom T. [1 ,2 ]
Hazarika D.J. [1 ]
Goswami G. [1 ]
Barooah M. [1 ]
Kalita M.C. [2 ]
Boro R.C. [1 ]
机构：
[1] Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
[2] Department of Biotechnology, Gauhati University, Guwahati, 781014, Assam
来源：
Indian Phytopathology | 2020年 / 73卷 / 1期
关键词：
5′ORF1a; Coat protein; CTV; Monomer structure; Recombination;
D O I：
10.1007/s42360-020-00199-x
中图分类号：
学科分类号：
摘要：
Citrus tristeza virus (CTV) is associated with three manifestations: citrus decline, stem pitting and seedling yellows of almost all citrus species and hybrids. Infected citrus plants harbour a large number of distinct CTV strains due to multiple and frequent inoculations by aphids and the fallible nature of its RNA polymerase. As such, it is important and necessary to identify different strains of CTV for proper management and disease control. In this study, we report the identification of 15 CTV isolates prevalent in three states of Northeast (NE) India: Assam, Meghalaya and Nagaland. A total of 50 samples were collected, out of which 15 showed CTV infection based on enzyme linked immuno-sorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) assays. RT-PCR products of 674 bp and 407 bp corresponding to the coat protein (CP) gene and 5′ORF1a region respectively were sequenced and their phylogenetic analysis was carried out. Analysis of putative recombination events detected recombination in four isolates in the 5′ORF1a fragment and in one isolate in the CTV-CP gene. Furthermore, CP monomer structure of four CTV isolates was predicted by Phyre2 online structure prediction tool. Phylogenetic studies involving more number of CTV isolates reveal better understanding of the recombination or evolutionary pattern of the virus which would aid in developing advanced CTV diagnostic methods. © 2020, Indian Phytopathological Society.
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页码：145 / 153
页数：8
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