Detection and quantification of onion isolates of Fusarium oxysporum f. sp. cepae in onion plant

被引：0
|
作者：
Kazunori Sasaki
Katsuya Nakahara
Masayoshi Shigyo
Shuhei Tanaka
Shin-ichi Ito
机构：
[1] Tottori University,The United Graduate School of Agricultural Sciences
[2] Yamaguchi University,Department of Biological and Environmental Sciences, Faculty of Agriculture
来源：
Journal of General Plant Pathology | 2015年 / 81卷
关键词：
Fusarium basal rot;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Fusarium oxysporum f. sp. cepae (FOC) isolated from onion basal rot possessed a SIX3 homolog (FocSIX3) with 91.4 % identity to the SIX3 gene (FolSIX3) of F. oxysporum f. sp. lycopersici (FOL). A primer pair (P1) was designed to detect onion FOC based on differences in nucleotide sequences between FocSIX3 and FolSIX3. P1 amplified a 106-bp-long DNA segment only from FOC isolates pathogenic to onions, but not from FOC isolated from either Welsh onion or F. oxysporum strains belonging to formae speciales other than cepae. Real-time quantitative PCR with P1 was developed to quantify onion root- and basal-plate-colonizing FOC.
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页码：232 / 236
页数：4
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