A trypsin inhibitor was isolated from Cassia obtusifolia by ammonium sulfate precipitation, Sepharose 4B-trypsin affinity and Sephadex G-75 chromatography. The inhibitor consisted of a single polypeptide chain with a molecular mass of 19, 812.55 Da. It was stable from pH 2 to 12 for 24 h, whereas it was unstable either above 70°C for 10 min or under reduced conditions. The inhibitor, which inhibited trypsin activity with an apparent Ki of 0.3 μM, had one reactive site involving a lysine residue. The native inhibitor was resistant to pepsin digestion, whereas the heated inhibitor produced 40% degree of susceptibility. The disulfide linkage and lysine residue were important in maintaining its conformation. Partial amino acid sequence of the purified protein showed a high degree of homology with various members of the Kunitz inhibitor family. Moreover, the inhibitor showed significant inhibitory activity against trypsin-like proteases present in the larval midgut on Pieris rapae and could suppress the growth of larvae.
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Zhou, Jiayu
Li, Chaolin
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Li, Chaolin
Chen, Anqi
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Chen, Anqi
Zhu, Jianquan
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Zhu, Jianquan
Zou, Meng
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Zou, Meng
Liao, Hai
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
Liao, Hai
Yu, Yamei
论文数: 0引用数: 0
h-index: 0
机构:
Sichuan Univ, West China Hosp, Canc Ctr, Chengdu 610041, Sichuan, Peoples R China
Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Sichuan, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Liu, Zubi
Zhu, Qiankun
论文数: 0引用数: 0
h-index: 0
机构:
Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Zhu, Qiankun
Li, Juanjuan
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Li, Juanjuan
Zhang, Gan
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Zhang, Gan
Jiamahate, Aerguli
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Jiamahate, Aerguli
Zhou, Jiayu
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Zhou, Jiayu
Liao, Hai
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Liu, Zubi
Zhu, Qiankun
论文数: 0引用数: 0
h-index: 0
机构:
Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Zhu, Qiankun
Li, Yangyang
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Li, Yangyang
Yu, Jihua
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Yu, Jihua
Wang, Wanjun
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Wang, Wanjun
Tan, Rui
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Tan, Rui
Zhou, Jiayu
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China
Zhou, Jiayu
Liao, Hai
论文数: 0引用数: 0
h-index: 0
机构:
Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R ChinaSouthwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China