A rapid co-culture stamping device for studying intercellular communication

被引：0
作者：
Amin Hassanzadeh-Barforoushi
Jonathan Shemesh
Nona Farbehi
Mohsen Asadnia
Guan Heng Yeoh
Richard P. Harvey
Robert E. Nordon
Majid Ebrahimi Warkiani
机构：
[1] School of Mechanical and Manufacturing Engineering,Department of Engineering
[2] University of New South Wales,Developmental and Stem Cell Biology Division
[3] Graduate School of Biomedical Engineering,undefined
[4] University of New South Wales,undefined
[5] Faculty of Science,undefined
[6] Macquarie University,undefined
[7] Victor Chang Cardiac Research Institute,undefined
[8] Sydney,undefined
[9] NSW,undefined
[10] 2010; St. Vincent’s Clinical School and School of Biotechnology and Biomolecular Science,undefined
[11] University of New South Wales,undefined
[12] Australian Centre for Nanomedicine,undefined
[13] University of New South Wales,undefined
[14] Sydney,undefined
[15] NSW 2052,undefined
[16] Australia; Garvan Institute of Medical Research,undefined
[17] Darlinghurst,undefined
[18] School of Medical Sciences,undefined
[19] Edith Cowan University,undefined
来源：
Scientific Reports | / 6卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of cultured cells, enabling analysis of co-culture boundary conditions on cell fate. We also developed an in-vitro model of endothelial and cardiac stem cell interactions, which are thought to regulate coronary repair after myocardial injury. The stamp is fabricated using microfabrication techniques, is operated with a lab pipettor and uses very low reagent volumes of 20 μl with cell injection efficiency of >70%. This easy-to-use device provides a general strategy for micro-patterning of multiple cell types and will be important for studying cell-cell interactions in a multitude of applications.
引用
收藏
相关论文
共 92 条
  • [11] Pan Z(2013)Optoacoustic tweezers: a programmable, localized cell concentrator based on opto-thermally generated, acoustically activated, surface bubbles Lab on a Chip 13 1772-1779
  • [12] Scadden DT(2012)Tunable patterning of microparticles and cells using standing surface acoustic waves Lab on a Chip 12 2491-2497
  • [13] Yeon JH(2009)Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW) Lab on a Chip 9 2890-2895
  • [14] Ryu HR(2008)Alignment of particles in microfluidic systems using standing surface acoustic waves Applied Physics Letters 92 044104-5548
  • [15] Chung M(1999)Patterning cells and their environments using multiple laminar fluid flows in capillary networks Proceedings of the National Academy of Sciences 96 5545-2347
  • [16] Hu QP(2005)A General Method for Patterning Gradients of Biomolecules on Surfaces Using Microfluidic Networks Analytical Chemistry 77 2338-12547
  • [17] Jeon NL(2002)Gradients of substrate-bound laminin orient axonal specification of neurons Proceedings of the National Academy of Sciences 99 12542-5185
  • [18] Bhowmick NA(2012)Targeted isolation and analysis of single tumor cells with aptamer-encoded microwell array on microfluidic device Lab on a Chip 12 5180-2376
  • [19] Neilson EG(1999)Patterning proteins and cells using soft lithography Biomaterials 20 2363-313
  • [20] Moses HL(1997)Using Microcontact Printing to Pattern the Attachment of Mammalian Cells to Self-Assembled Monolayers of Alkanethiolates on Transparent Films of Gold and Silver Experimental Cell Research 235 305-4248
12345678910