Evaluation of Chimerism in DNA Samples by PCR Amplification of D1S80 With Detection by Capillary Electrophoresis

被引：0
作者：
Cyrenius M. Jone
Nahida Akel
Anthony A. Killeen
机构：
[1] University of Michigan,Department of Pathology
来源：
Molecular Diagnosis | 2000年 / 5卷 / 2期
关键词：
PCR; bone marrow transplantation; chimerism detection; D1S80; capillary electrophoresis;
D O I：
10.1007/BF03262028
中图分类号：
学科分类号：
摘要：
Background: Analysis of the relative amounts of donor and recipient DNA in bone marrow after bone marrow transplantation is frequently used to determine the statusof the transplant. We studied the performance of an assay to quantify chimerism based on amplification of the D1S80 variable number tandem repeat marker by PCR with detection of PCR products by capillary electrophoresis (CE).
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页码：101 / 105
页数：4
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