Backbone 1H, 13C and 15N resonance assignments of dengue virus NS2B–NS3p in complex with aprotinin

被引：0
作者：
Yunchen Bi
Lei Zhu
Hua Li
Bo Wu
Jinsong Liu
Junfeng Wang
机构：
[1] Chinese Academy of Sciences,High Magnetic Field Laboratory, Hefei Institutes of Physical Science
[2] Hefei Institutes of Physical Science,Center of Medical Physics and Technology
[3] Chinese Academy of Sciences,Guangzhou Institutes of Biomedicine and Health
[4] Chinese Academy of Sciences,undefined
来源：
Biomolecular NMR Assignments | 2013年 / 7卷
关键词：
Dengue virus; Complex; NS2B–NS3p; Inhibitor; Assignment; NMR;
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摘要：
Dengue virus, belongs to Flaviviridae, is an arthropod transmitted virus that threatens millions of people’s lives. As with other flaviviruses, a positive single-stranded 11-kilobases RNA in the dengue virus genome encodes three structural proteins (capsid protein C, membrane protein M, and envelope protein E) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The two component protease NS2B–NS3p is essential for viral replication and is believed to be a potential antiviral drug target. Aprotinin, a native inhibitor, is proved to retard the activity of NS2B–NS3p. The backbone assignments of NS2B–NS3p will be essential for determining the high resolution solution structure of NS2B–NS3p and screening new antiviral drugs. Herein, we report the backbone 1H, 15N, 13C resonance assignments of the N terminal fragment of NS2B (4.8 kDa) and NS3p (18.5 kDa) in complex with aprotinin (6.5 kDa) by high resolution NMR.
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页码：137 / 139
页数：2
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