Capillary electrophoresis determination of diatrizoic acid and its impurities in diatrizoate radiopaque solutions

被引:0
|
作者
Shawky A. Farag
Clyde E. Wells
机构
[1] Food and Drug Administration,Division of Drug Analysis
来源
Microchimica Acta | 1997年 / 126卷
关键词
capillary electrophoresis; diatrizoate; separation; radiopaque; impurities; degradation;
D O I
暂无
中图分类号
学科分类号
摘要
A capillary electrophoresis method has been developed for the separation and determination of diatrizoic acid (DTZA) and its four mono- and diiodo degradation products (2-iodo,4-iodo, 2,4-diiodo, and 2,6-diiodo-3,5-diacetamidobenzoic acid) in radio-paque solution for injection (RSI). DTZA and its degradants were assayed in suitable dilutions without pretreatment. Optimum conditions included the use of low-pressure sample injection (6 s), sample and standard solutions with a molarity less than that of the separation buffer, and adjustment of the buffer molarity to obtain a current of 50 μA at a constant 15kV separation voltage. After each six runs or less, the inlet and outlet buffer were replaced with more buffer from the same batch. When the method was applied to a sample of SI levels of 5–10 mg/ml were found for each of the mono and diiodo impurities. The optimum method showed a precision (peak area measurement) in the range 1.7–7.2% RSD, depending on the concentration. A linear correlation coefficient of 0.997 was obtained over a DTZA concentration range of 5–60 mg/mL.
引用
收藏
页码:141 / 145
页数:4
相关论文
共 50 条
  • [21] Determination of mycophenolic acid and its glucuronide conjugate in human plasma by capillary electrophoresis
    S. Ünsalan
    G. Hempel
    J. Boos
    G. Blaschke
    Chromatographia, 2001, 54 : 635 - 637
  • [22] Determination of oleanolic acid in Ligustrum lucidum and its medicinal preparation by capillary electrophoresis
    Liu, HT
    Wang, KT
    Chen, XG
    Hu, ZD
    ANALYTICAL LETTERS, 2000, 33 (06) : 1105 - 1115
  • [23] Determination of aristolochic acid by capillary zone electrophoresis
    Kvasnicka, F
    Sevcík, R
    Voldrich, M
    Krátká, J
    CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, 2004, 2 (03): : 417 - 424
  • [24] Capillary electrophoresis for the determination of bromide, chloride and sulfate as impurities in calcium acamprosate
    Fabre, H
    Blanchin, MD
    Bosc, N
    ANALYTICA CHIMICA ACTA, 1999, 381 (01) : 29 - 37
  • [25] Determination of acid dissociation constants by capillary electrophoresis
    Poole, SK
    Patel, S
    Dehring, K
    Workman, H
    Poole, CF
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1037 (1-2) : 445 - 454
  • [26] DETERMINATION OF IMPURITIES IN A NOVEL ANALOG OF ADENOSINE 5'-TRIPHOSPHATE BY CAPILLARY ELECTROPHORESIS
    DAWSON, JR
    NICHOLS, SC
    TAYLOR, GE
    JOURNAL OF CHROMATOGRAPHY A, 1995, 700 (1-2) : 163 - 172
  • [27] Capillary zone electrophoresis method for the determination of famotidine and related impurities in pharmaceuticals
    Helali, N.
    Tran, N. T.
    Monser, L.
    Taverna, M.
    TALANTA, 2008, 74 (04) : 694 - 698
  • [28] Determination of thiodiglycolic acid in urine by capillary electrophoresis
    Samcová, E
    Kvasnicová, V
    Urban, J
    Jelínek, I
    Coufal, P
    JOURNAL OF CHROMATOGRAPHY A, 1999, 847 (1-2) : 135 - 139
  • [29] Determination of Fluorobenzoic Acid by Capillary Zone Electrophoresis
    Liu Tao
    Guo Ming
    Meng Liang
    Fu Xiao-Qin
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2010, 38 (07) : 994 - 998
  • [30] Determination of thioglycolic acid in cosmetics by capillary electrophoresis
    Xie, Na
    Ding, Xiaojing
    Wang, Xinyu
    Wang, Ping
    Zhao, Shan
    Wang, Zhi
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2014, 88 : 509 - 512