Induction, regeneration, and biolistic sensitivities of different genotypes of common wheat (Triticum aestivum L.)

被引：0
作者：
V. S. Fadeev
H. R. Shimshilashvili
A. K. Gaponenko
机构：
[1] Russian Academy of Sciences,Vavilov Institute of General Genetics
[2] Russian Academy of Sciences,Center of Bioengineering
[3] Russian Academy of Sciences,Kol’tsov Institute of Developmental Biology
来源：
Russian Journal of Genetics | 2008年 / 44卷
关键词：
Common Wheat; Cold Treatment; Immature Embryo; High Molecular Weight Glutenin Subunit; Biolistic Transformation;
D O I：
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中图分类号：
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摘要：
The induction, regeneration, and biolistic sensitivities of different genotypes of common wheat (Triticum aestivum L.) have been determined in order to develop an efficient system for transformation of Russian cultivars of spring wheat. Short-term (two days) cold treatment (4°C) has been demonstrated to distinctly increase the frequency of morphogenetic callus induction. The optimal phytohormonal composition of the nutrient medium ensuring an in vitro regeneration rate of the common wheat cultivar Lada as high as 90% has been determined. The optimal temporal parameters of genetic transformation of wheat plants (10–14 days of culturing after initiation of a morphogenetic callus) have been determined for two transformation methods: biolistic without precipitated DNA and transformation with the plasmid psGFP-BAR. Analysis of the transient expression of the gfp gene has confirmed that 14 days of culturing is the optimal duration.
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页码：1096 / 1104
页数：8
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