Easy-to-Use Aids to a New Unifying Approach to Quantitative Luminescence Microscopy

Work has already been reported indicating the attractions of certain easy-to-use, essentially stable, light standards, in forms suited to testing and calibrating the quantitative photometry of a wide variety of (chemi-)luminometers. Appropriate log/log plots of such calibrations not only offer great insight into the photometric performance of such instruments, but also often permit direct comparisons of the analytical results from the various instruments concerned. More recently, this approach has been extended to an analogous evaluation of simple fluorimeters, and this paper reports further application of the approach to fluoresence microscopy.