Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha

[1] Brakhage AA(1998)Molecular regulation of beta-lactam biosynthesis in filamentous fungi Microbiol Mol Biol Rev 62 547-585

[2] van de Kamp M(1999)Compartmentalization and transport in beta-lactam antibiotic biosynthesis by filamentous fungi Antonie Van Leeuwenhoek 75 41-78

[3] Driessen AJ(2002)delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine synthetase, that mediates the first committed step in penicillin biosynthesis, is a cytosolic enzyme Fungal Genet Biol 37 49-55

[4] Konings WN(1995)Amino-acid substitutions in the cleavage site of acyl-coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum: effect on proenzyme cleavage and activity Gene 162 29-35

[5] van der Lende TR(2007)Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes Chem Biol 14 329-339

[6] van de Kamp M(1999)The reaction cycle of isopenicillin N synthase observed by X-ray diffraction Nature 401 721-724

[7] Berg M(1995)Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes Nature 375 700-704

[8] Sjollema K(2007)Interactions of isopenicillin N synthase with cyclopropyl-containing substrate analogues reveal new mechanistic insight Biochemistry 46 4755-4762

[9] Bovenberg RA(1999)Recent advances in the structure and function of isopenicillin N synthase Antonie Van Leeuwenhoek 75 33-39

[10] Veenhuis M(2007)Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene FEMS Yeast Res 7 1160-1167

[1] Brakhage AA(1998)Molecular regulation of beta-lactam biosynthesis in filamentous fungi Microbiol Mol Biol Rev 62 547-585

[2] van de Kamp M(1999)Compartmentalization and transport in beta-lactam antibiotic biosynthesis by filamentous fungi Antonie Van Leeuwenhoek 75 41-78

[3] Driessen AJ(2002)delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine synthetase, that mediates the first committed step in penicillin biosynthesis, is a cytosolic enzyme Fungal Genet Biol 37 49-55

[4] Konings WN(1995)Amino-acid substitutions in the cleavage site of acyl-coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum: effect on proenzyme cleavage and activity Gene 162 29-35

[5] van der Lende TR(2007)Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes Chem Biol 14 329-339

[6] van de Kamp M(1999)The reaction cycle of isopenicillin N synthase observed by X-ray diffraction Nature 401 721-724

[7] Berg M(1995)Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes Nature 375 700-704

[8] Sjollema K(2007)Interactions of isopenicillin N synthase with cyclopropyl-containing substrate analogues reveal new mechanistic insight Biochemistry 46 4755-4762

[9] Bovenberg RA(1999)Recent advances in the structure and function of isopenicillin N synthase Antonie Van Leeuwenhoek 75 33-39

[10] Veenhuis M(2007)Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene FEMS Yeast Res 7 1160-1167