Identification and Antimicrobial Activity of Phenylacetic Acid Produced by Bacillus licheniformis Isolated from Fermented Soybean, Chungkook-Jang

被引:0
作者
Yoon Kim
Jeong-Yong Cho
Ju-Hee Kuk
Jae-Hak Moon
Jeong-Il Cho
Young-Cheol Kim
Keun-Hyung Park
机构
[1] Department of Food Science & Technology,
[2] and Institute of Agricultural Science & Technology,undefined
[3] Chonnam National University,undefined
[4] Gwangju 500-757,undefined
[5] Department of Applied Biology and Chemistry. The Research Institute of Agricultural Resources Development,undefined
[6] Konkuk University,undefined
[7] Seoul 143-701,undefined
[8] Division of Food Science,undefined
[9] Chosun College of Science and Technology,undefined
[10] Gwangju 501-744,undefined
[11] Division of Applied Plant Science,undefined
[12] Chonnam National University,undefined
[13] Gwangju 500-757,undefined
来源
Current Microbiology | 2004年 / 48卷
关键词
Fermentation; Antimicrobial Activity; Candida; Candida Albicans; Culture Filtrate;
D O I
暂无
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学科分类号
摘要
A bacterial strain, B65-1, which showed strong antimicrobial activity, was isolated from Chungkook-Jang, a traditional Korean fermented-soybean food with antimicrobial properties. Based on carbon utilization pattern and partial 16S rRNA sequence analysis, the B65-1 strain was identified as Bacillus licheniformis. An antibiotic compound, active against bacteria and yeast such as Staphylococcus aureus, Escherichia coli, and Candida albicans, was isolated by various chromatographic procedures from culture filtrates of B. licheniformis B65-1. The purified antibiotic was identified to be phenylacetic acid, with the molecular formula C8H8O2 by analyses of EI-MS and NMR. The phenylacetic acid was detected in fermented soybean made with the strain B65-1 as a starter, but was not present in extracts of nonfermented soybean. Our results indicated that the phenylacetic acid produced by B. licheniformis during fermentation of soybean is one of the main compounds of antimicrobial activity of Chungkook-Jang.
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页码:312 / 317
页数:5
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