Efficient validation of single nucleotide polymorphisms in plants by allele-specific PCR, with an example from barley

被引：0
|
作者：
V. D. Soleimani
B. R. Baum
D. A. Johnson
机构：
[1] Agriculture and Agri-Food Canada (AAFC) Central Experimental Farm,Ottawa
[2] University of Ottawa,Carleton Institute of Biology
来源：
Plant Molecular Biology Reporter | 2003年 / 21卷
关键词：
allele-specific PCR; SNP validation;
D O I：
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摘要：
Although the increasing number of expressed sequence tags (ESTs) from the public domain has facilitated the detection of single nucleotide polymorphisms (SNPs), further validation is needed before they can be used as markers. For SNP validation, we have compared 2 independent methods: (1) the primer extension method followed by capillary electrophoresis on an ABI PRISM 3100 Genetic Analyzer and (2) nested PCR followed by agarose-based visualization. We present an assessment of the efficiency and costs associated with these methods, based on a sample of barley cultivars.
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页码：281 / 288
页数：7
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