Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids

被引：0
|
作者：
Oscar Rodríguez
Raquel O. Cristóvão
Ana P. M. Tavares
Eugénia A. Macedo
机构：
[1] University of Porto,LSRE–Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering
来源：
Applied Biochemistry and Biotechnology | 2011年 / 164卷
关键词：
Ionic liquids; Laccase; Stability; Activity; Kinetic parameters;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C4mim][Cl], 1-octyl-3-methyl imidazolium chloride [C8mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C10mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C4mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C10mim][Cl] > [C8mim][Cl] > [C4mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis–Menten parameters. Among the ILs, [C4mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.
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页码：524 / 533
页数：9
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