Isolation and characterization of a novel glycogen synthase kinase-3 gene, GmGSK, in Glycine max L. that enhances abiotic stress tolerance in Saccharomyces cerevisiae

被引：0

作者：

Chunbao Zhang

Hongkun Zhao

Yanzhi Liu

Qiyun Li

Xiaodong Liu

Hua Tan

Cuiping Yuan

Yingshan Dong

机构：

[1] Northeast Normal University,College of Life Science

[2] Jilin Academy of Agricultural Sciences,Biotechnology Research Center

来源：

Biotechnology Letters | 2010年 / 32卷

关键词：

Abiotic stress; Drought tolerance; Glycogen synthase kinase-3; Salt tolerance; Subcellular localization;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A novel glycogen synthase kinase-3 gene, GmGSK, was isolated from Glycinemax. It is 1,596 bp in length with one ORF of 410 amino acids. Southern blot analysis revealed that it has at least two copies in the G. max genome. GmGSK, when transiently expressed in Nicotiana tabacum leaves, was localized in both cell membrane and cytoplasm. Northern blot analysis indicated that GmGSK is expressed in all tissues, with highest expression in the root. GmGSK can be induced by various abiotic stresses. When transformed with GmGSK, Saccharomyces cerevisiae exhibited enhanced resistance to salt and drought stress.

引用

页码：861 / 866

页数：5

共 38 条

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