Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model

被引：0
作者：
Han Zhang
Yue Teng Wei
Kam Sze Tsang
Chong Ran Sun
Jin Li
Hua Huang
Fu Zhai Cui
Yi Hua An
机构：
[1] Capital Medical University,Beijing Neurosurgical Institute
[2] Tsinghua University,Department of Materials Science and Engineering
[3] Chinese University of Hong Kong,Department of Anatomical and Cellular Pathology
[4] Chinese University of Hong Kong,Li Ka Shing Institute of Health Sciences
[5] Second Affiliated Hospital of Zhejiang University Medical College,Department of Neurosurgery
来源：
Journal of Translational Medicine | / 6卷
关键词：
Nerve Fiber; Hyaluronic Acid; Facial Nerve; Myelin Sheath; Muscular Atrophy;
D O I：
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学科分类号：
摘要：
The implantation of neural stem cells (NSCs) in artificial scaffolds for peripheral nerve injuries draws much attention. NSCs were ex-vivo expanded in hyaluronic acid (HA)-collagen composite with neurotrophin-3, and BrdU-labeled NSCs conduit was implanted onto the ends of the transected facial nerve of rabbits. Electromyography demonstrated a progressive decrease of current threshold and increase of voltage amplitude in de-innervated rabbits after implantation for one, four, eight and 12 weeks compared to readouts derived from animals prior to nerve transection. The most remarkable improvement, observed using Electrophysiology, was of de-innervated rabbits implanted with NSCs conduit as opposed to de-innervated counterparts with and without the implantation of HA-collagen, NSCs and HA-collagen, and HA-collagen and neurotrophin-3. Histological examination displayed no nerve fiber in tissue sections of de-innervated rabbits. The arrangement and S-100 immunoreactivity of nerve fibers in the tissue sections of normal rabbits and injured rabbits after implantation of NSCs scaffold for 12 weeks were similar, whereas disorderly arranged minifascicles of various sizes were noted in the other three arms. BrdU+ cells were detected at 12 weeks post-implantation. Data suggested that NSCs embedded in HA-collagen biomaterial could facilitate re-innervations of damaged facial nerve and the artificial conduit of NSCs might offer a potential treatment modality to peripheral nerve injuries.
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