Measurement of homonuclear three-bond J(HNHα) coupling constants in unlabeled peptides complexed with labeled proteins: Application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV)

被引：0
作者：
Daniel O. Cicero
Gaetano Barbato
Uwe Koch
Paolo Ingallinella
Elisabetta Bianchi
Sonia Sambucini
Petra Neddermann
Raffaele De Francesco
Antonello Pessi
Renzo Bazzo
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来源：
Journal of Biomolecular NMR | 2001年 / 20卷
关键词：
coupling constants; HCV; HNHA; isotope filtering; NS3;
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摘要：
A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(HNHα) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the `quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV).
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页码：23 / 29
页数：6
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