Improved Drought and Salt Tolerance in Transgenic Nicotiana benthamiana by Overexpressing Sugarcane ScSEC14p Gene

被引：0
作者：
Yongjuan Ren
Huaying Mao
Weihua Su
Yachun Su
Chang Zhang
Mutian Shi
Youxiong Que
机构：
[1] Fujian Agriculture and Forestry University,Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, College of Agriculture
[2] Fujian Agriculture and Forestry University,Key Laboratory of Ministry of Education for Genetic, Breeding and Multiple Utilization of Crops, College of Agriculture
[3] Fujian Agriculture and Forestry University,College of Horticulture
来源：
Sugar Tech | 2021年 / 23卷
关键词：
Phosphatidylinositol transfer proteins (PITPs); Drought tolerance; Salt tolerance; Osmotic stress; Transgenic tobacco;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Plant phosphatidylinositol transfer proteins (PITPs) are involved in several cellular processes such as lipid metabolism, signal transduction, osmotic regulation, protein transport and stress response. In this study, the effects of sugarcane PITP gene ScSEC14p (accession no. MH899751) on drought and salt tolerance of transgenic Nicotiana benthamiana were evaluated along with the underlying mechanisms. The results showed that ScSEC14p gene effectively improved drought and salt tolerance of transgenic lines of N. benthamiana, as indicated by the increased germination rate and superoxide dismutase levels, as well as the decreased malondialdehyde level. In addition, the enhanced tolerance was closely related to the upregulation of stress-related genes and key genes in phosphatidylinositol pathway. It is revealed that the overexpression of ScSEC14p gene resulted in improved drought and salt tolerance of transgenic tobacco and also elucidated the underlying regulation mechanism by ScSEC14p in sugarcane.
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页码：326 / 335
页数：9
相关论文
共 242 条
[1]  
Agnieszka KM(2016)Expression and characterization of a barley phosphatidylinositol transfer protein structurally homologous to the yeast Sec14p protein Plant Science 246 98-111
[2]  
Ewa B(2018)Epigenetic responses to abiotic stresses during reproductive development in cereals Plant Reproduction 31 343-355
[3]  
Anna TT(1989)Inositol phosphates and cell signalling Nature 341 197-205
[4]  
Pascal R(2015)The cotton WRKY gene PLoS ONE 10 e0143022-109
[5]  
Tadeusz R(2006) positively regulates salt and drought stress tolerance in transgenic Plant Growth Regulation 48 97-3679
[6]  
Begcy K(2019)Phospholipids as plant growth regulators Frontiers in Plant Science 9 2003-308
[7]  
Dresselhaus T(2008), a sucrose non-fermenting-related protein kinase gene, positively regulates plant response to drought and salt stress in transgenic tobacco African Journal of Biotechnology 7 3677-1231
[8]  
Berridge MJ(2011) culture techniques as a tool of sugarcane bud germination study under salt stress BioFactors 37 290-50
[9]  
Irvine RF(1985)Phosphatidylinositol transfer proteins: negotiating the regulatory interface between lipid metabolism and lipid signaling in diverse cellular processes Science 227 1229-1382
[10]  
Chu XQ(2016)A simple and general method for transferring genes into plants Frontiers in Plant Science 7 548-4017
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