Techniques for detecting protein-protein interactions in living cells: principles, limitations, and recent progress

被引:0
作者
Yaning Cui
Xi Zhang
Meng Yu
Yingfang Zhu
Jingjing Xing
Jinxing Lin
机构
[1] Beijing Forestry University,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design
[2] Beijing Forestry University,College of Biological Sciences & Biotechnology
[3] Henan University,Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology
来源
Science China Life Sciences | 2019年 / 62卷
关键词
protein-protein interactions; techniques; RET; PCA; co-localization;
D O I
暂无
中图分类号
学科分类号
摘要
Detecting protein-protein interactions (PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET (smFRET), FRET-fluorescence lifetime imaging microscopy (FRET-FLIM), cytoskeleton-based assay for protein-protein interaction (CAPPI) and single-molecule protein proximity index (smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.
引用
收藏
页码:619 / 632
页数:13
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