Two-pore-domain potassium channels support anion secretion from human airway Calu-3 epithelial cells

被引:0
作者
Kellie A. Davis
Elizabeth A. Cowley
机构
[1] Dalhousie University,Department of Physiology and Biophysics
来源
Pflügers Archiv | 2006年 / 451卷
关键词
Transepithelial anion secretion; Epithelial K; channels; Cystic fibrosis; Airway epithelia;
D O I
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中图分类号
学科分类号
摘要
Potassium channels are required for the absorption and secretion of fluids and electrolytes in epithelia. Calu-3 cells possess a secretory phenotype, and are a model human airway submucosal gland serous cell. Short-circuit current (Isc) recordings from Calu-3 cells indicated that basal anion secretion was reduced by apical application of the K+ channel inhibitors bupivicaine, lidocaine, clofilium, and quinidine. Application of riluzole resulted in a large increase in Isc, inhibited by apical application of either bupivicane or the cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel blocker DPC. These results suggested that one or more members of the two-pore-domain K+ (K2P) channel family could influence anion secretion. Using RT-PCR, we found that Calu-3 cells express mRNA transcripts for TASK-2 (KCNK5), TWIK-1 (KCNK1), TWIK-2 (KCNK6) and TREK-1 (KCNK2). TASK-2, TWIK-2 and TREK-1 protein were detected by Western blotting, while immunolocalization of polarized cells confirmed protein expression of TREK-1 and TWIK-2 at the plasma cell membrane. TASK-2 protein staining was localized to intracellular vesicles, located beneath the apical membrane. While the pro-secretory role of basolateral K+ channels is well established, we suggest that apically located K2Pchannels, not previously described in airway epithelial cells, also play an important role in controlling the rate of transepithelial anion secretion.
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页码:631 / 641
页数:10
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