Analysis of female salivary gland proteins of the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand

被引：0
作者：
Narissara Jariyapan
Visut Baimai
Yong Poovorawan
Sittiruk Roytrakul
Atiporn Saeung
Sorawat Thongsahuan
Sittiporn Suwannamit
Yasushi Otsuka
Wej Choochote
机构：
[1] Chiang Mai University,Department of Parasitology, Faculty of Medicine,
[2] Mahidol University,Department of Biology and Center for Vectors and Vector
[3] Chulalongkorn University,Borne Diseases, Faculty of Science,
[4] National Science and Technology Development Agency,Department of Pediatric, Faculty of Medicine,
[5] Oita University,National Center for Genetic Engineering and Biotechnology (BIOTEC),
来源：
Parasitology Research | 2010年 / 107卷
关键词：
Sodium Dodecyl Sulfate; Salivary Gland; Sibling Species; Lateral Lobe; Blood Feeding;
D O I：
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中图分类号：
学科分类号：
摘要：
Electrophoretic protein profiles of female salivary glands of five sibling species within the Anopheles barbirostris complex, namely A. barbirostris species A1 (Forms A, B, and D), A2, A3, and A4 and Anopheles campestris-like (Forms B and E), were analyzed. At least eight major and several minor protein bands were detected in the glands of each species, of which each morphological region contained different major proteins. The protein profiles distinguished the five sibling species. The variability in major proteins among species was observed in the 40–48, 32–37, and 10–18 kDa ranges. No difference in protein profiles was found in different cytogenetic forms. Polymorphism of the protein profiles within species was only noted in species A4. The lowest major protein (marker) band of each species showed remarkably different relative mobility on SDS–polyacrylamide gels. NanoLC-MS analysis revealed that the marker protein of some species matched with a protein involving in blood feeding, gSG6, of Anopheles gambiae and Anopheles freeborni. These results might be useful for construction of an additional tool to distinguish the five sibling species and lead to further study on the evolution of blood feeding and pathogen transmission.
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页码：509 / 516
页数：7
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  • [1] Apiwathnasorn C(2002)Potential for J Med Entomol 39 583-586
  • [2] Prommongkol S(1999) (Diptera: Culicidae) to transmit malaria parasites in Pa Rai subdistrict (Aranyaprathet, Sa Kaeo province), Thailand Parasitologia 41 483-487
  • [3] Samung Y(1999)Salivary gland-specific gene expression in the malaria vector Proc Natl Acad Sci U S A 96 1516-1521
  • [4] Limrat D(2005)Trapping cDNAs encoding secreted proteins from the salivary glands of the malaria vector J Exp Biol 208 3971-3986
  • [5] Rojruthai B(1995)An updated catalogue of salivary gland transcripts in the adult female mosquito, J Am Mosq Control Assoc 11 323-328
  • [6] Arca B(1995)Metaphase karyotypes of Am J Trop Med Hyg 53 478-481
  • [7] Lombardo F(2000) of Thailand and Southeast Asia: IV. The barbirostris and umbrosus species groups, subgenus Proc Natl Acad Sci U S A 97 13859-13865
  • [8] Capurro M(2004) (Diptera: Culicidae) Insect Mol Biol 13 73-88
  • [9] della Torre A(2007)Discrimination of all members of the Insect Biochem Mol Biol 37 164-175
  • [10] Spanos L(2009) complex by polymerase chain reaction-restriction fragment length polymorphism analysis BMC Genomic 10 57-209
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