Virtual slit scanning microscopy

被引：0

作者：

Reto Fiolka

Andreas Stemmer

Yury Belyaev

机构：

[1] ETH Zurich,Nanotechnology Group

[2] European Molecular Biology Laboratory,Advanced Light Microscopy Facility

来源：

Histochemistry and Cell Biology | 2007年 / 128卷

关键词：

Confocal microscopy; Microscopy; Scanning microscopy;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We present a novel slit scanning confocal microscope with a CCD camera image sensor and a virtual slit aperture for descanning that can be adjusted during post-processing. A very efficient data structure and mathematical criteria for aligning the virtual aperture guarantee the ease of use. We further introduce a method to reduce the anisotropic lateral resolution of slit scanning microscopes. System performance is evaluated against a spinning disk confocal microscope on identical specimens. The virtual slit scanning microscope works as the spinning disk type and outperforms on thick specimens.

引用

页码：499 / 505

页数：6

共 5 条

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[2]

Minsky undefined(1988)Memoir on inventing the confocal scanning microscope Scanning 10 128-138

[3]

Rector undefined(2003)High-performance confocal system for microscopic or endoscopic applications Methods 30 16-27

[4]

Sheppard undefined(1988)Confocal Microscopes with Slit Apertures J Mod Opt 35 1169-1185

[5]

Verveer undefined(1997)Theory of confocal fluorescence imaging in the programmable array microscope J Microsc Oxf 189 192-198

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