Melatonin protects against myocardial hypertrophy induced by lipopolysaccharide

被引:0
|
作者
Qi Lu
Xin Yi
Xiang Cheng
Xiaohui Sun
Xiangjun Yang
机构
[1] The First Affiliated Hospital of Soochow University,Department of Cardiology
[2] The First Affiliated Hospital of Nantong University,Department of Cardiology
[3] Nantong university,Department of Human Anatomy, Medical College
[4] Nantong Elderly Rehabilitation Hospital,undefined
[5] Soochow University,undefined
来源
In Vitro Cellular & Developmental Biology - Animal | 2015年 / 51卷
关键词
Myocardial hypertrophy; Melatonin; Lipopolysaccharide; Tumor necrosis factor-α; Ca; overload;
D O I
暂无
中图分类号
学科分类号
摘要
Melatonin is thought to have the ability of antiatherogenic, antioxidant, and vasodilatory. It is not only a promising protective in acute myocardial infarction but is also a useful tool in the treatment of pathological remodeling. However, its role in myocardial hypertrophy remains unclear. In this study, we investigated the protective effects of melatonin on myocardial hypertrophy induced by lipopolysaccharide (LPS) and to identify their precise mechanisms. The cultured myocardial cell was divided into six groups: control group, LPS group, LPS + ethanol (4%), LPS + melatonin (1.5 mg/ml) group, LPS + melatonin (3 mg/ml) group, and LPS + melatonin (6 mg/ml) group. The morphologic change of myocardial cell was observed by inverted phase contrast microscope. The protein level of myocardial cell was measured by Coomassie brilliant blue protein kit. The secretion level of tumor necrosis factor-α (TNF-α) was evaluated by enzyme-linked immunosorbent assay (ELISA). Ca2+ transient in Fura-2/AM-loaded cells was measured by Till image system. The expression of Ca2+/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) was measured by Western blot analysis. Our data demonstrated that LPS induced myocardial hypertrophy, promoted the secretion levels of TNF-α, and increased Ca2+ transient level and the expression of CaMKII and CaN. Administration of melatonin 30 min prior to LPS stimulation dose-dependently attenuated myocardial hypertrophy. In conclusion, the results revealed that melatonin had the potential to protect against myocardial hypertrophy induced by LPS in vitro through downregulation of the TNF-α expression and retains the intracellular Ca2+ homeostasis.
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页码:353 / 360
页数:7
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