5-Aminosalicylic Acid Protection against Oxidative Damage to Synaptosomal Membranes by Alkoxyl Radicals In Vitro

被引：0
作者：
Jaroslaw Kanski
Christopher Lauderback
D. Allan Butterfield
机构：
[1] University of Kentucky,Department of Chemistry, Center of Membrane Sciences, and Sanders
来源：
Neurochemical Research | 2001年 / 26卷
关键词：
Reactive oxygen species; EPR; 5-aminosalicylic acid; (5-ASA), 2,2′-azobis(amino propane) dihydrochloride; (AAPH); azo-bis(isobutyronitrile); (AIBN); synaptosomal membranes; antioxidants;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The antioxidant properties of 5-aminosalicylic acid in vitro were evaluated in a synaptosomal membrane system prepared from gerbil cortical synaptosomes using EPR spin labeling and spectroscopic techniques. MAL-6 (2,2,6,6-tetramethyl-4-maleimidopiperidin-1-oxyl) and 5-NS (5-nitroxide stearate) spin labels were used to assess changes in protein oxidation and membrane lipid fluidity, respectively. Synaptosomal membranes were subjected to oxidative stress by incubation with 1 mM azo-bis(isobutyronitrile) (AIBN) or 1 mM 2,2′-azobis(amidino propane) dihydrochloride (AAPH) at 37°C for 30 minutes. The EPR analyses of the samples showed significant oxidation of synaptosomal proteins and a decrease in membrane fluidity. 5-Aminosalicylic acid also was evaluated by means of FRAP (the ferric reducing ability of plasma) test as a potential antioxidant. 5-Aminosalicylic acid also showed protection against the oxidation in gerbil cortical synaptosomes system caused by AIBN and AAPH. These results are consistent with the notion of antioxidant protection against free radical induced oxidative stress in synaptosomal membrane system by this agent.
引用
收藏
页码：23 / 29
页数：6
相关论文
共 174 条
[1]  
Markesbery W. R.(1997)Oxidative stress hypothesis in Alzeimer disease Free Rad. Biol. Med. 23 134-147
[2]  
Varadarajan S.(2000)Review: Alzheimer's amyloid β-peptide-associated free radical oxidative stress and neurotoxicity J. Struct. Biol. 130 184-208
[3]  
Yatin S.(1998)Elevated “hydroxyl radical” generation in vivo in an animal model of amyotrophic lateral sclerosis J. Neurochem. 71 1321-1324
[4]  
Aksenova M.(1992)Protein oxidation and aging Science 257 1224-871
[5]  
Butterfield D. A.(1997)4-hydroxy-2-nonenal pyrrole adducts in human neurodegenerative disease J. Neuropathol. Exp. Neurol. 56 866-1169
[6]  
Bogdanov M. B.(1997)The lipid peroxidation product, 4-hydroxy-2-trans-nonenal, alters the conformation of cortical synaptosomal membrane proteins J. Neurochem. 69 1161-317
[7]  
Ramos L.E.(1999)Peroxynitrite-induced alterations in synaptosomal membrane proteins: insight into oxidative stress in Alzheimer's disease J. Neurochem. 72 310-331
[8]  
Xu Z.(1994)Electron paramagnetic resonance investigations of free radical induced alterations in neurocortical synaptosomal membrane protein infrastructure Free Rad. Biol. Med. 17 321-506
[9]  
Beal M.F.(1997)Beta-Amyloid-associated free radical oxidative stress and neurotoxicity: implications for Alzhemier's disease Chem. Res. Toxicol. 10 495-586
[10]  
Stadtman E.R.(1996)Oxidation of vitamin E, vitamin C, and thiols in rat brain synaptosomes by peroxynitrite Biochem. Pharmacol. 52 579-157
12345678910