Streptomyces lividans and Brevibacterium lactofermentum as heterologous hosts for the production of X22 xylanase from Aspergillus nidulans

被引:0
作者
M. Díaz
S. A. I. Adham
D. Ramón
J. A. Gil
R. I. Santamaría
机构
[1] Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca,Instituto de Microbiología Bioquímica/Departamento de Microbiología y Genética
[2] Universidad de León,Departamento de Ecología, Genética y Microbiología, Area de Microbiología, Facultad de Biología
[3] University of Waterloo,Department of Biology
[4] Universitat de Valencia,Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia
[5] Instituto de Agroquímica y Tecnología de Alimentos (CSIC),Departamento de Biotecnología
来源
Applied Microbiology and Biotechnology | 2004年 / 65卷
关键词
Streptomyces; Xylanase Activity; Corynebacterium; Aspergillus Nidulans; Clear Halo;
D O I
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学科分类号
摘要
The Aspergillus nidulans gene xlnA coding for the fungal xylanase X22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.
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页码:401 / 406
页数:5
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