Biofilm Inhibitory Effect of Spirulina platensis Extracts on Bacteria of Clinical Significance

被引：14

作者：

LewisOscar F. ^{[1
]}

Nithya C. ^{[1
]}

Bakkiyaraj D. ^{[1
]}

Arunkumar M. ^{[1
]}

Alharbi N.S. ^{[2
]}

Thajuddin N. ^{[1
,2
]}

机构：

[1] Division of Microbial Biodiversity and Bioenergy, Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu

[2] Department of Botany and Microbiology, College of Science, King Saud University, Riyadh

来源：

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences | 2017年 / 87卷 / 2期

关键词：

Antibiofilm; Biofilm; Cell surface hydrophobicity; Extracellular polymeric substances; S; platensis;

D O I：

10.1007/s40011-015-0623-9

中图分类号：

学科分类号：

摘要：

Spirulina platensis is one of the most potential microalgae explored for antibacterial, antiviral and anti-cancerous properties. However, its antibiofilm potential has not been studied. Biofilms are of significant interest as they confer resistance towards antimicrobials and host immunity both in diverse group of bacteria. Exploring Spirulina towards the biofilm would give an easy way of treatment against bacterial pathogens. In this milieu, the antibiofilm potentials of organic extracts prepared from S. platensis was revealed. The results clearly showed that methanolic extract of S. platensis at a concentration of 100 ng mL−1 efficiently inhibited the biofilms of Vibrio parahaemolyticus (ATCC17802), Chromobacterium violaceum (ATCC 12742) and Vibrio alginolyticus (ATCC17749) about 90, 89 and 88 % respectively. Significant reduction in cell surface hydrophobicity was documented for Aeromonas hydrophila (MTCC1739), Escherichia coli (MTCC 739) and Staphylococcus aureus (MTCC 96 and 2940). Besides the inhibition of extracellular polymeric substances in A. hydrophila (MTCC1739) and S. aureus (MTCC2940) was about 88 and 71 % respectively. The availability of Spirulina as nutritious food makes it as a foremost contender against bacterial biofilm. The present study reveals the antibiofilm potential of S. platensis against a broad spectrum of both Gram Positive and Gram Negative bacteria. S. platensis effectively inhibited the biofilm of Vibro spp., a major menace in aquaculture industries. Further characterization and purification of the active compounds could be a major remedy against biofilm forming bacteria. © 2015, The National Academy of Sciences, India.

引用

页码：537 / 544

页数：7

共 37 条

[1]

Costerton J.W., Lewandowski Z., DeBeer D., Caldwell D., Korber D., James G., Biofilms, the customized micro niche, J Bacteriol, 176, pp. 2137-2142, (1994)

[2]

Wingender J., Strathmann M., Rode A., Leis A., Flemming H.C., Isolation and biochemical characterization of extracellular polymeric substances from Pseudomonas aeruginosa, Methods Enzymol, 336, pp. 302-314, (2001)

[3]

Whitchurch C.B., Tolker-Nielsen T., Ragas P.C., Mattick J.S., Extracellular DNA required for bacterial biofilm formation, Science, 295, (2002)

[4]

Kolenbrander P.E., Palmer R.J., Human oral bacterial biofilms, Microbial biofilms, (2004)

[5]

Hoiby N., Doring G., Schiotz P.O., The role of immune complexes in the pathogenesis of bacterial infections, Annu Rev Microbiol, 40, pp. 29-53, (1986)

[6]

Bjarnsholt T., Jensen P.O., Fiandaca M.J., Pedersen J., Hansen C.R., Andersen C.B., Pressler T., Givskov M., Hoiby N., Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients, Pediatr Pulmonol, 44, pp. 547-558, (2009)

[7]

Hall-Stoodley L., Hu F.Z., Gieseke A., Nistico L., Nguyen D., Hayes J., Forbes M., Greenberg D.P., Dice B., Burrows A., Wackym P.A., Stoodley P., Post J.C., Ehrlich G.D., Kerschner J.E., Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media, JAMA, 296, pp. 202-211, (2006)

[8]

Dutta D., Cole N., Willcox M., Factors influencing bacterial adhesion to contact lenses, Mol Vis, 18, pp. 14-21, (2012)

[9]

Li Y.H., Tang N., Aspiras M.B., Lau P.C.Y., Lee J.H., Ellen R.P., Cvitkovitch D.G., A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation, J Bacteriol, 184, pp. 2699-2708, (2002)

[10]

Merritt J., Qi F., Goodman S.D., Anderson M.H., Shi W., Mutation of luxS affects biofilm formation in Streptococcus mutans, Infect Immun, 71, pp. 1972-1979, (2003)

← 1 2 3 4 →