High-performance liquid chromatography ganglioside pattern of the AB feline blood group

被引：5
作者：
Silvestre-Ferreira A.C. [1 ]
Masso O. [2 ]
Pastor J. [3 ]
机构：
[1] Veterinary Sciences Department, Veterinary Hospital, University of Trás-os-Montes e Alto Douro, Vila Real
[2] Merck Farma y Química, S.A. Parc Científic de Barcelona, Barcelona
[3] Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, Barcelona
来源：
Comparative Clinical Pathology | 2011年 / 20卷 / 6期
关键词：
Blood types; Blood typing; Feline; HPLC; TLC;
D O I：
10.1007/s00580-010-1039-9
中图分类号：
学科分类号：
摘要：
Feline blood types A, B, and AB are known for several years. However, the exact mode of inheritance of type AB blood is still unknown. The use of Triticum vulgaris lectin and the anti-A serum is by far the most common method for feline blood typing. T. vulgaris lectin is known to identify a sialoglycoprotein in type B and AB animals, and it also recognizes N-acetylneuraminic acid gangliosides derivates. On the other hand, anti-A serum recognizes N-glycolylneuraminic acid derivates. In the present work, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and TLC immunostaining with monoclonal antibodies P3 and 14F9 were used. N-acetylneuraminic and N-glycolylneuraminic derivate gangliosides were confirmed as major determinants in feline blood types However, by the use of HPLC, six ganglioside patterns were found. Type B cats showed only one pattern, but type A animals revealed the presence of three distinct patterns. Serological type AB cats presented two patterns that overlap with the three from type A cats. In conclusion, ganglioside patterns from A and AB blood types cats diverged from the serological classification. © 2010 Springer-Verlag London Limited.
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页码：597 / 605
页数：8
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