Identification of salt-stress responsive genes in rice (Oryza sativa L.) by cDNA array

被引：0
作者：
Xinjian He
Jianquan Chen
Zhigang Zhang
Jinsong Zhang
Shouyi Chen
机构：
[1] Chinese Academy of Sciences,Institute of Genetics and Developmental Biology
来源：
Science in China Series C: Life Sciences | 2002年 / 45卷
关键词：
cDNA array; stress; salt responsive gene; rice;
D O I：
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学科分类号：
摘要：
To identify salt stress-responsive genes, we constructed a cDNA library with the salttolerant rice cultivar, Lansheng. About 15000 plasmids were extracted and dotted on filters with Biomeck 2000 HDRT system or by hand. Thirty genes were identified to display altered expression levels responding to 150 mmol/L NaCl. Among them eighteen genes were up-regulated and the remainders downregulated. Twenty-seven genes have their homologous genes in GenBank Databases. The expression of twelve genes was studied by Northern analysis. Based on the functions, these genes can be classified into five categories, including photosynthesis-related gene, transportrelated gene, metabolismrelated gene, stress-or resistancerelated gene and the others with various functions. The results showed that salt stress influenced many aspects of rice growth. Some of these genes may play important roles in plant salt tolerance.
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页码：477 / 484
页数：7
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