Experimental infection of mice with Borna disease virus (BDV): Replication and distribution of the virus after intracerebral infection

被引：0
作者：
Enbergs H.K. [1 ]
Vahlenkamp T.W. [1 ]
Kipar A. [1 ]
Müller H. [1 ]
机构：
[1] Institute of Virology, University of Leipzig, D-04103 Leipzig
来源：
Journal of NeuroVirology | 2001年 / 7卷 / 3期
关键词：
BDV; CNS; Mouse; PBMC;
D O I：
10.1080/13550280152403317
中图分类号：
学科分类号：
摘要：
To develop an animal model resembling natural asymptomatic Borna disease virus (BDV) infections, BDV He/80 rat brain homogenate was passaged four times in adult SJL/J mice. Within 12 months of observation, mice did not develop overt signs of disease. Nucleotide sequencing of the rat isolate and the mouse isolates at the fourth passage revealed no difference in the deduced amino acids. Viral RNA was found in brain, heart, kidney, lung, liver, and urinary bladder. Infectious virus was isolated from brain, but also from heart and lung tissue. Immunohistochemically, BDV was demonstrated in nerves in the abdominal cavity, ganglion coeliacum, and adrenal glands, but not in organ parenchyma. Occasionally, viral RNA was detected in mononuclear blood cells.
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页码：272 / 277
页数：5
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