Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

被引：0
|
作者：
Outhiriaradjou Benard
K.A. Balasubramanian
机构：
[1] Christian Medical College and Hospital,The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences
来源：
Molecular and Cellular Biochemistry | 1997年 / 170卷
关键词：
GSH; butyrate; BSO; HT-29 cells; proliferation; differentiation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.
引用
收藏
页码：109 / 114
页数：5
相关论文
共 50 条
  • [31] Underlying mechanism for the modulation of apoptosis induced by a new benzoindole derivative on HT-29 colon cancer cells
    Hajiaghaalipour, Fatemeh
    Bagheri, Elham
    Faraj, Fadhil Lafta
    Abdulla, Mahmood Ameen
    Majid, Nazia Abdul
    RSC ADVANCES, 2017, 7 (61): : 38257 - 38263
  • [32] Induction of apoptosis by phloretin in HT-29 human colon cancer cells
    Park, SY
    Kim, EJ
    Shin, HK
    Kwon, DY
    Surh, YJ
    Park, JHY
    FASEB JOURNAL, 2006, 20 (04): : A568 - A568
  • [33] Modulation of CYP3A4 expression by ceramide in human colon carcinoma HT-29 cells
    Chun, YJ
    Lee, S
    Yang, SA
    Park, S
    Kim, MY
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 298 (05) : 687 - 692
  • [34] REGULATION OF VITAMIN-D RECEPTOR ABUNDANCE AND RESPONSIVENESS DURING DIFFERENTIATION OF HT-29 HUMAN COLON CANCER-CELLS
    ZHAO, X
    FELDMAN, D
    ENDOCRINOLOGY, 1993, 132 (04) : 1808 - 1814
  • [35] Modulation of HMG-N2 binding to chromatin by butyrate-induced acetylation in human colon adenocarcinoma cells
    Lührs, H
    Hock, R
    Schauber, J
    Weihrauch, M
    Harrer, M
    Melcher, R
    Scheppach, W
    Bustin, M
    Menzel, T
    INTERNATIONAL JOURNAL OF CANCER, 2002, 97 (05) : 567 - 573
  • [36] DCC regulates cell adhesion in human colon cancer derived HT-29 cells and associates with ezrin
    Martin, Merce
    Simon-Assmann, Patricia
    Kedinger, Michele
    Martin, Marianne
    Mangeat, Paul
    Real, Francisco X.
    Fabre, Myriam
    EUROPEAN JOURNAL OF CELL BIOLOGY, 2006, 85 (08) : 769 - 783
  • [37] The stable isotope-based dynamic metabolic profile of butyrate-induced HT29 cell differentiation
    Boren, J
    Lee, WNP
    Bassilian, S
    Centelles, JJ
    Lim, S
    Ahmed, S
    Boros, LG
    Cascante, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (31) : 28395 - 28402
  • [38] Sodium Butyrate Induces Human Colon Carcinoma HT-29 Cell Apoptosis Through a Mitochondrial Pathway
    Wang, L.
    Luo, H-S
    Xia, H.
    JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, 2009, 37 (03) : 803 - 811
  • [39] Inflammation-Induced Down-Regulation of Butyrate Oxidation Can Be Counteracted in HT-29 Cells by Butyrate
    Boesmans, Leen
    De Preter, Vicky
    Verbeke, Kristin
    GASTROENTEROLOGY, 2013, 144 (05) : S832 - S832
  • [40] Effects of differentiation on purinergic and neurotensin-mediated calcium signaling in human HT-29 colon cancer cells
    Chowdhury, Mohammad A.
    Peters, Amelia A.
    Roberts-Thomson, Sarah J.
    Monteith, Gregory R.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 439 (01) : 35 - 39
12345