Microscopic and molecular detection of Nosema spp. in honeybees of Turkey

被引:0
作者
Armagan Erdem Utuk
Fatma Cigdem Piskin
Ahmet Onur Girisgin
Ozgur Selcuk
Levent Aydin
机构
[1] Cukurova University,Faculty of Ceyhan Veterinary Medicine, Department of Parasitology
[2] Central Veterinary Control and Research Institute,Parasitology and Bee Diseases Laboratory
[3] Uludag University,Faculty of Veterinary Medicine, Department of Parasitology
来源
Apidologie | 2016年 / 47卷
关键词
Multiplex PCR; Turkey;
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摘要
In this study, we aimed to determine the prevalence of Nosema spp. in honeybees of Turkey. For this aim, adult honeybee (Apis mellifera) samples were collected from 1621 colonies within 95 apiaries located in 22 provinces of Turkey. Samples were examined microscopically. In case of positivity, spore identification was done by multiplex PCR. At the end of microscopic examination, Nosema spp. spores were detected in 7 out of 22 provinces (31.8 %), and 16 out of 95 colonies (16.8 %) that represent 1621 colonies. According to PCR results, 1 out of 16 isolates (6.25 %) was Nosema apis, and 15 out of 16 isolates (93.75 %) were Nosema ceranae. The result of our study indicated that N.ceranae is the dominant species in Turkey.
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页码:267 / 271
页数:4
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